Plasmid_Backbone

Part:BBa_K4129025

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-06)


PAC572: plasmid for transient expression in Aspergillus niger.

This is a plasmid for transient expression in Aspergillus niger containing an E.coli ori, an ampicillin selection marker, a PyrG auxotrophyc selection marker and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7180
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 8018
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7180
    Illegal NheI site found at 8674
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 8018
    Illegal NotI site found at 1296
    Illegal NotI site found at 8665
    Illegal NotI site found at 8719
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7180
    Illegal BglII site found at 7082
    Illegal BamHI site found at 7220
    Illegal BamHI site found at 7756
    Illegal XhoI site found at 3619
    Illegal XhoI site found at 4336
    Illegal XhoI site found at 4814
    Illegal XhoI site found at 7616
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7180
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 8018
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3078
    Illegal EcoRI site found at 5355
    Illegal EcoRI site found at 7180
    Illegal XbaI site found at 1257
    Illegal XbaI site found at 2023
    Illegal XbaI site found at 2505
    Illegal XbaI site found at 3273
    Illegal XbaI site found at 5160
    Illegal XbaI site found at 5928
    Illegal XbaI site found at 6410
    Illegal SpeI site found at 1440
    Illegal SpeI site found at 1451
    Illegal SpeI site found at 1815
    Illegal SpeI site found at 1947
    Illegal SpeI site found at 2071
    Illegal SpeI site found at 2178
    Illegal SpeI site found at 2189
    Illegal PstI site found at 4222
    Illegal PstI site found at 8018
    Illegal NgoMIV site found at 4356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6949
    Illegal BsaI site found at 7809
    Illegal BsaI.rc site found at 1484


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